codon optimized superfolder gfp Search Results


codon-optimized superfolder green fluorescent protein gene sfgfp  (Basler)
90
Basler codon-optimized superfolder green fluorescent protein gene sfgfp
Codon Optimized Superfolder Green Fluorescent Protein Gene Sfgfp, supplied by Basler, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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codon optimized superfolder gfp  (Addgene inc)
93
Addgene inc codon optimized superfolder gfp
Codon Optimized Superfolder Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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codon optimized superfolder gfp  (Bio Basic Canada)
86
Bio Basic Canada codon optimized superfolder gfp
Codon Optimized Superfolder Gfp, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superfolder-(sf)gfp  (Sandia Biotech)
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Sandia Biotech superfolder-(sf)gfp
Superfolder (Sf)gfp, supplied by Sandia Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human superfoods from e. gracilis  (Euglena Co Ltd)
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Euglena Co Ltd human superfoods from e. gracilis
Human Superfoods From E. Gracilis, supplied by Euglena Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superfolder gfp  (GenScript corporation)
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GenScript corporation superfolder gfp
Superfolder Gfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superfolder gfp/product/GenScript corporation
Average 90 stars, based on 1 article reviews
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superfolder gfp sgfp  (Twist Bioscience)
86
Twist Bioscience superfolder gfp sgfp
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Superfolder Gfp Sgfp, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
superfolder gfp sgfp - by Bioz Stars, 2026-06
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gfp gene, superfolder gfp  (GenScript corporation)
90
GenScript corporation gfp gene, superfolder gfp
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Gfp Gene, Superfolder Gfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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plasmid encoding tele with the c terminus fused with superfolder gfp  (GenScript corporation)
90
GenScript corporation plasmid encoding tele with the c terminus fused with superfolder gfp
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Plasmid Encoding Tele With The C Terminus Fused With Superfolder Gfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid encoding tele with the c terminus fused with superfolder gfp/product/GenScript corporation
Average 90 stars, based on 1 article reviews
plasmid encoding tele with the c terminus fused with superfolder gfp - by Bioz Stars, 2026-06
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optimized gfp gene, superfolder gfp  (GenScript corporation)
90
GenScript corporation optimized gfp gene, superfolder gfp
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Optimized Gfp Gene, Superfolder Gfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optimized gfp gene, superfolder gfp/product/GenScript corporation
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poly-his (mghhhhhhgsgsg)-monomeric superfolder (msf)egfp  (GenScript corporation)
90
GenScript corporation poly-his (mghhhhhhgsgsg)-monomeric superfolder (msf)egfp
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Poly His (Mghhhhhhgsgsg) Monomeric Superfolder (Msf)egfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly-his (mghhhhhhgsgsg)-monomeric superfolder (msf)egfp/product/GenScript corporation
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cyclized circular permuted superfolder-gfp (cgfp) coding sequence  (GenScript corporation)
90
GenScript corporation cyclized circular permuted superfolder-gfp (cgfp) coding sequence
Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of <t>sGFP</t> (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.
Cyclized Circular Permuted Superfolder Gfp (Cgfp) Coding Sequence, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cyclized circular permuted superfolder-gfp (cgfp) coding sequence/product/GenScript corporation
Average 90 stars, based on 1 article reviews
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Image Search Results


Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of sGFP (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.

Journal: mSphere

Article Title: Analysis of Teg41 and PSMα promoter activity using a divergent fluorescent reporter plasmid

doi: 10.1128/msphere.00432-25

Figure Lengend Snippet: Dual reporter plasmid assay reveals two different activity profiles for the Teg41 and psmα promoters. ( A ) Schematic of the dual reporter plasmid (pPRB4) constructed. The psmα promoter drives expression of sGFP (green), while the Teg41 promoter controls expression of mCherry (red). A translation initiation region (TIR, yellow) was added before the start codon of mCherry to initiate translation. ( B ) S. aureus AH1263 carrying pPRB4 was grown in TSB with chloramphenicol (10 µg/mL) at 37°C, and fluorescence intensities for both sGFP (green) and mCherry (red), as well as OD 600 (black), were monitored over 16 h. The maximum fluorescence intensity for each fluorophore was set at 100%. Data are represented as the average of three replicates ± standard deviation (SD). ( C ) Relationship between fluorescence intensity and bacterial growth for both the Teg41 (right axis) and psmα promoters (left axis). Raw fluorescence data were plotted against bacterial OD 600 . ( D and E ) Plasmid pPRB4 was phage-transduced into different S. aureus backgrounds: AH1263 (blue), NCTC-8325 (red), TCH1516 (green), Newman (purple), JE2 (orange), COL (black), Mu50 (brown), and UAMS-1 (dark blue). Strains were grown under similar conditions as in panel B. Promoter activities for Teg41 ( D ) and psmα ( E ) were calculated as described in Materials and Methods. Values were normalized to 100% for AH1263 ± SD. **** P < 0.0001 by analysis of variance corrected with Dunnett’s multiple comparison test.

Article Snippet: Superfolder GFP (sGFP), mCherry (codon optimized for S. aureus ), and the promoter sequence of psmα-Teg41 (268 bp) were synthesized by Twist Bioscience.

Techniques: Plasmid Preparation, Activity Assay, Construct, Expressing, Fluorescence, Standard Deviation, Comparison

Teg41 promoter activity is relatively stable in the absence of global regulatory proteins. ( A and B ) Plasmid pPRB4 was phage-transduced into S. aureus AH1263 (WT), AH1263 Teg41Δ3′, or AH1263 agrA ::ery strains, and the fluorescence intensities of sGFP ( A ) and mCherry ( B ) were recorded as proxies for psmα and Teg41 promoter activities, respectively. Fluorescence intensity is expressed as a percentage relative to the WT strain, which was set at 100%. ( C ) Teg41 expression in five transcriptional regulator mutants from RNA-seq. Results are shown as log2 fold change in expression compared to the WT strain in each experiment. Dotted lines represent log2FC >1 or <−1. ( D ) Plasmid pPRB4 was phage-transduced into S. aureus JE2 WT (blue), rot ::ery (red), hpf ::ery (green), or sarA ::ery (black), and Teg41 promoter activity was monitored as previously described. Promoter activity is expressed as a percentage relative to the JE2 WT strain, which was set at 100% ± SD.

Journal: mSphere

Article Title: Analysis of Teg41 and PSMα promoter activity using a divergent fluorescent reporter plasmid

doi: 10.1128/msphere.00432-25

Figure Lengend Snippet: Teg41 promoter activity is relatively stable in the absence of global regulatory proteins. ( A and B ) Plasmid pPRB4 was phage-transduced into S. aureus AH1263 (WT), AH1263 Teg41Δ3′, or AH1263 agrA ::ery strains, and the fluorescence intensities of sGFP ( A ) and mCherry ( B ) were recorded as proxies for psmα and Teg41 promoter activities, respectively. Fluorescence intensity is expressed as a percentage relative to the WT strain, which was set at 100%. ( C ) Teg41 expression in five transcriptional regulator mutants from RNA-seq. Results are shown as log2 fold change in expression compared to the WT strain in each experiment. Dotted lines represent log2FC >1 or <−1. ( D ) Plasmid pPRB4 was phage-transduced into S. aureus JE2 WT (blue), rot ::ery (red), hpf ::ery (green), or sarA ::ery (black), and Teg41 promoter activity was monitored as previously described. Promoter activity is expressed as a percentage relative to the JE2 WT strain, which was set at 100% ± SD.

Article Snippet: Superfolder GFP (sGFP), mCherry (codon optimized for S. aureus ), and the promoter sequence of psmα-Teg41 (268 bp) were synthesized by Twist Bioscience.

Techniques: Activity Assay, Plasmid Preparation, Fluorescence, Expressing, RNA Sequencing